"I could not... have had this life and lived it with the level of intensity that is required to do this show the way it's done," Winfrey said. "I'd be one of those people that the kid's coming and saying, 'Mom, you've neglected me.'... So I have no regrets about that. I have none ... not one regret about not having children because I believe that ... it is the way it's supposed to be."
Winfrey will end her appointment with 6.5 million viewers a day when her namesake show, broadcast in 145 countries, comes to an end in May. She will shift her focus onto a new venture, the launch of her network, OWN, The Oprah Winfrey Network, which debuts in January and will feature a combination of in-depth documentaries and reality shows featuring Winfrey and others.
While OWN's debut will mark the realization of another career goal for the media mogul, it's a shift into uncharted territory.
"At first, I thought, 'Great, a network. Oh, gee, this is the dream I had.' And ... as that started to settle in with me, I thought, 'Whoa! What is this I've gotten myself into? This is a lot more work than I ever imagined,'" Winfrey told Walters.
Winfrey said that taking on a whole new network is frightening.
"I was very scared ... I would wake up in the middle of the night literally like clutching my chest, like, 'What have I done? What have I done?'" she recalled.
Watch the interview on "A Barbara Walters Special: Oprah, The Next Chapter," Thursday at 9 p.m. ET