Peter Sloan, 59, filed the civil suit in Hillsborough County Court, seeking monetary compensation, as well as a DNA test to prove Sloan is the son of the legendary actor.
Sloan, who is a radio DJ, claims his late mother, actress Kathy McNeil, had an affair with Shatner before he was born.
Sloan claims Shatner, 85, admitted in 1984 on the set of "T.J. Hooker" that he was his father, but the actor has denied the claim, according to the court documents obtained by ABC News. Furthermore, Sloan claims that a representative for Shatner contacted him and told him not to go public with his claims, the documents state.
Shatner's rep and legal team had no comment to ABC News on the lawsuit. His legal team has also not yet responded in court to the civil suit.
Sloan is seeking $30 million in compensatory damages, $90 million in punitive damages and $50 million in pain and suffering. He is also seeking that Shatner stop claiming he is not his son.
In a letter given to ABC News by Sloan, Erik Hyman, Shatner's legal representative, asserts in 2011, "I had another conversation with Bill, who confirmed to me once again that he is not your father. There have been many people over the years who have claimed to be his children or other relatives."
Sloan further claims in the court docs that Shatner's team has slandered him in the press and on social media.
Sloan is also suing IMDbPro and Twitter because he claims they eliminated his connections to Shatner.
"When I look at his face, I see myself in many ways," Sloan told "Good Morning America" in a segment that aired this morning. "I'll be darned if I'll be called a liar and that's what it really boils down to."