A third person, Rudy Guede, 22, was sentenced to 30 years in prison after being convicted of murder in October 2008, in a short-track trial that allowed him to avoid a life sentence. All three young people say they did not kill Kercher.
Kercher's body was found Nov. 2, 2007, in the house she shared with Knox in the university town of Perugia. She was found partly undressed and died from a combination of knife wounds to her throat and strangulation, according to coroners.
In his final remarks, Mignini told the judges and jurors that he had "gotten to know Amanda Knox fairly well" in the course of his interrogation of her and during the trial.
"And I have observed her at length in court," Mignini said.
In his description of Knox's personality, Mignini said he was quoting an evaluation of her by a well-known criminologist. When he read the evaluation, Mignini said he was struck by how it matched his impression of the accused killer.
According to this unidentified criminologist, Knox also has a tendency to develop a dislike for people who do not agree with her, and her feelings are "anesthetized."
Sollecito's profile also fit, Mignini said, when it described him as someone who depended on others for approval, a "dependent personality." Mignini added that he also found Sollecito to be cold.
Neither defendant reacted visibly to the prosecutor's description of them.
Just before the final remarks, the court was shown a 20-minute video reconstruction made by investigators, showing what they believe was the dynamic of the murder.
Using Google maps of the town of Perugia, and computer generated models resembling Knox, Sollecito, Guede and Kercher superimposed on actual photos or digital re-creations of the scene of the crime, the video retraced events from the hours before the murder through to its aftermath.
The visual representation reinforced what Mignini had described in his reconstruction of the crime on the previous day. Autopsy photos were also included to illustrate how the injuries found on the victim's bodies were produced by the action of the three alleged killers.
Both Knox and Sollecito wielded knives, in this reconstruction, while Guede sexually molested Kercher.
Knox did not watch the video, sitting quietly in court and staring straight ahead. Sollecito did watch, without showing any reaction.
Most of this second day of closing arguments was taken up by Mignini's fellow prosecutor Manuela Comodi, who focused on the complex and controversial forensic and technical evidence in the case
Addressing the jury at the end, Mignini said, "When this moment comes, the biggest mistake is to look only at the defendants and not what they are accused of, and at the victim. This is when you should be remembering her.
"A young woman was killed for no real reason, just days before going home to visit her ailing mother. She was killed in a horrific manner, and now all her family can do is gather at the cemetery. She was literally eliminated forever," the prosecutor told jurors.