"You don't expect to give somebody tens of billions of dollars and say, 'Don't tell me how you're going to spend it,'" she said.
"Things will be different," Pelosi said of the second round of TARP spending, "because we will have a president who will enforce the law, and with the light of transparency, that will be built into any new law -- if there is to be any more TARP funding."
Democrats, who control Congress, also seek assurances that a sizable amount of the money will be allocated to help homeowners avoid foreclosure. In the House, Democrats are drafting legislation that would dedicate a minimum of $40 billion to that effort.
The TARP money is just a downpayment on bailout funds the Obama administration will ask Congress to approve. The president-elect's economic team is hammering out a massive stimulus plan that will include tax cuts, bailout funds and public works projects expected to cost around $775 billion.
Obama is scheduled to travel to a factory in Ohio Friday in an effort to highlight the need for the massive stimulus package.
Together with the TARP request, Obama seeks more than $1 trillion to help stabilize the nation's economy.
ABC News' Zach Wolf contributed to this report.