"Republicans used to be for transparency," the vice president told ABC News' Terry Moran on Tuesday.
"I'm not taking their word for it," Biden said of the Chamber's assertion that foreign money was not paying for political advertising.
Last week the left-leaning group MoveOn.org called on the Democrats who have received the Chamber's help to condemn the ads and ask that the television spots be taken down.
Maryland Rep. Frank Kratovil, a Democrat who is in a tight race with Republican State Sen. Andrew Harris in the state's 1st Congressional District, has no plans to renounce the Chamber, a campaign spokeswoman said on Tuesday.
"At the end of the day it shows that Frank is an independent leader," Kratovil's campaign manager Jessica Klonsky said of the ad. Kratovil's campaign website prominently touts his Chamber of Commerce endorsement.
In Idaho, conservative Democratic Rep. Walt Minnick's campaign manager, John Foster, said the Chamber's ads "are about Walt's work in the district." The campaign has no plans to ask that they be taken off the air.
"Walt has always maintained his focus on the district first and put a lot less importance on what kind of political intrigue is happening in Washington, D.C.," Foster said in an interview. "We're a long way from the Hill."
And despite the volley of attacks from the White House, the Democratic National Committee and other party affiliates, Democratic leaders have yet to call on the Chamber to remove the ads.
White House spokesman Josh Earnest emphasized that President Obama's criticism of outside groups influencing the election, particularly the Chamber, was about transparency in the political system more than partisan politics, even though so much of the money is benefitting the GOP.
"The president believes it poses a threat to our democracy for a group like the Chamber to spend tens of millions of dollars, from undisclosed sources, in an effort to influence American elections," Earnest said, "regardless of the candidate they are supporting."