Madonna attends the World Premiere of "The Beatles: Eight Days A Week - The Touring Years" at Odeon Leicester Square, Sept. 15, 2016 in London.

Madonna rides her bike in Mayfair, April 18, 2016, in London.

Madonna and Sean Penn arrives at the 5th Annual Sean Penn & Friends HELP HAITI HOME Gala Benefiting J/P Haitian Relief Organization at Montage Hotel on Jan. 9, 2016 in Beverly Hills, Calif.

Madonna posted this photo of herself to Instagram, Nov. 25, 2014.

Madonna posted this photo on Instagram on March 21, 2014 with the caption, "Long hair...... Don't Care!!!!!! #artforfreedom #rebelheart #revolutionoflove".

Madonna is seen on crutches on her way to the gym in New York City, Jan. 16, 2014.

As Madonna prepares to release her new film, "W.E." she sat down with Cynthia McFadden for a revealing interview. Watch "Nightline" on Thursday night, "Good Morning America" on Friday morning and "20/20" for more on Madonna...And don't forget to tweet #AskMadonna to be part of the conversation!

During the interview, Cynthia McFadden asked Madonna several questions submitted by viewers. <a href="http://abcnews.go.com/Entertainment/video/madonna-personal-abc-news-exclusive-15326209">Click here to watch a preview. </a>

Madonna leaves little to the imagination in the Fall 2010 Dolce & Gabbana ad campaign. The 52-year-old wears low-cut bodysuit while channelling a sexy housewife for the photo. Of Madonna being the face of the campaign, Domenico Dolce and Stefano Gabbana founders said, "To have Madonna in our campaign is a dream come true."

At 51, Madonna rocks the cover and inside pages of Interview magazine. The ever-fit Queen of Pop dishes to film director, Gus Van Sant on early inspirations, recent film projects and trips to Malawi.

"I go to Malawi twice a year. It's where two of my children were adopted from, and I have a lot of projects there that I go and check up on and children who I look after," Madonna said in an interview with director Gus Van Sant. "It's sort of a commitment that I've made to this country and the hundreds of thousands of children there who have been orphaned by AIDS. I made a documentary about it ["I Am Because We Are," 2008], and it's just become part of my life."

On an early mentor, Christopher Flynn, who taught Madonna ballet, she told director Gus Van Sant in Interview magazine, "Growing up in Michigan, I didn't really know what a gay man was. He was the first man -- the first human being -- who made me feel good about myself and special. He was the first person who told me that I was beautiful or that I had something to offer the world, and he encouraged me to believe in my dreams, to go to New York. He was such an important person in my life."

Madonna's third greatest hits album, which came out on Sept. 29, 2009, featured hit titles that encompassed her career. With songs dating from 1982, this album features such classics as "Like a Virgin" and "Like a Prayer." The album is capped off with a brand new single "Celebration," which peaked at 71 on the Billboard Hot 100.

Madonna in a Shanghai restaurant in 1986, prior to the shooting of "Shanghai Surprise," which she starred in with then-husband Sean Penn.

A young Madonna, during simpler times. Here she prepares for her first communion in 1967. Her brother, Christopher Ciccone, writes in his 2008 book "Life With My Sister Madonna" that as a child, Madonna was "determined."