"I knew it was official this morning when NBC dropped off all my CDs and picked up its lava lamp," O'Brien joked on the show Thursday night.
After weeks of late-night controversy, played out publicly in the media, O'Brien will hit the door after seven months as show host with a reported $33 million payout to pave the way for former host Jay Leno to reclaim the seat at the beginning of March. O'Brien's staff is expected to receive $12 million in the severance package.
"I have to return the Etch A Sketch my contract was written on," O'Brien told his audience.
The settlement also allows O'Brien to return to the airwaves for another outlet as early as Sept. 1, 2010, although there has been little indication of where he'll end up.
The Fox network has been O'Brien's most vocal would-be suitor, but, O'Brien manager Gavin Polone said, there are no formal talks. There are "other possibilities" beyond Fox, he added, but the News Corp.-owned company is "certainly a terrific network."
Since word of the shift became public, O'Brien has been relentless in taking jabs at NBC during his show, which could soon change. The exit package is said to include a "nondisparagement" clause that would prevent O'Brien and NBC from saying negative things about one another for a set period of time.
Suspense has been building over how O'Brien will spend what is expected to be his final night at NBC. He joked on Tuesday's show that he was "just three days away from the biggest drinking binge in history."
"I am determined to make the best of the situation, so ladies and gentlemen here's the plan," O'Brien said Thursday to a roaring audience. "Tonight and tomorrow night we are going to have a lot of fun on television."
The show announced that Friday's guests would be Oscar-winner Tom Hanks and comic-actor Will Ferrell, who was O'Brien's first guest when he made his "Tonight Show" debut in June.
Hanks, who was originally slated to appear on the show Tuesday night, posted a message on his Twitter page about the upcoming appearance: "Flag on me with CoCo tonight! Going on Friday's Big Show. What WILL happen? Tune in. Hanx."
Nostalgic fans who will never see the show live can still buy tickets on eBay, where one Arizona watcher listed a "Tonight Show" ticket supposedly autographed by flame-haired O'Brien for $6,000. As of Thursday afternoon, no one had bid on it.
Payback: Conan Style
How do you get back at a boss who wronged you? How about spending millions of your boss's cash before you head out the door?
That has apparently been O'Brien's strategy during his final two shows in which he introduced the Bugatti Veyron Mouse, a $2 million Bugatti sports car adorned with fake mouse ears and whiskers Wednesday. Thursday's new character was far more expensive: 2009 Kentucky Derby winner Mine That Bird, wearing a mink Snuggie and watching restricted NFL Super Bowl footage.
"That's going to cost a pretty penny ... total pricetag: $4.8 million," O'Brien said gleefully.
O'Brien claimed Wednesday that featuring the Bugatti and the classic song "Satisfaction" on the show would cost NBC a total of $1.5 million.
O'Brien will be replaced by Leno, whose 10 p.m. prime-time show is being canceled after lagging ratings and complaints from NBC affiliates who said Leno's show provided a weak lead-in to their late newscasts.
"The good news is until NBC yanks us off the air, we can pretty much do whatever we want and, this is the best part, we can do whatever we want and they have to pay for it," O'Brien explained before introducing the car. "So for the rest of the week we're going to introduce new comedy bits that aren't so much funny as they are crazy expensive."
When it comes to the bit's price tag, however, the comedian may have been off by $1 million or so. New York-based entertainment lawyer Steve Gordon told ABCNews.com Thursday that existing agreements between NBC and music licensing companies would allow the "Tonight Show" to play "Satisfaction" at no additional cost for a live or time-delayed performance.
If the show were to be rerun, he said, NBC might have to pay $25,000 to $50,000 for the song's use to its owner, Abkco, which owns much of the Stones' early work. If a clip of the song were used on the Internet, he said, a similar or greater fee could apply, assuming Abkco allowed permission for its use.
As for the Bugatti -- O'Brien called it the most expensive car in the world, and he's likely right. Car research Web site Edmunds.com lists the price for the 2009 Bugatti Veyron model at $1.99 million.
But the show didn't actually shell out any money to put an earlier model of the Bugatti Veyron on air. The Petersen Automotive Museum in Los Angeles told ABCNews.com Thursday that it lent the show its 2006 Bugatti Veyron for free.
The show "contacted us and we decided it might be a good thing to help out," said museum information and marketing manager Chris Brown.
The car -- which, like the 2009 model, is valued at roughly $2 million -- had been in museum storage most recently but it is now back on display.
"Being we are as close as we are to Hollywood, we do like to have vehicles on the silver screen and small screen on display," Brown said. "To have a car like this that's recently been on TV, it's a good thing."
Even if "The Tonight Show" had to pay for the car, the price for its temporary use could be in the five-figure range. Luxury car rental service Beverly Hills Rent-a-Car, for instance, charges $25,000 per day for the rental of a Bugatti Veyron.
After weeks of wrangling between "Tonight Show" host O'Brien and NBC, the network announced Thursday that it and O'Brien are officially parting ways.
"Under terms of an agreement that was signed earlier today, NBC and O'Brien will settle their contractual obligations and the network will release O'Brien from his contract, freeing him to pursue other opportunities after September 1, 2010," NBC said.
The network said that Leno, who previously hosted the "Tonight Show" from May 1992 to May 2009, would return to the program on March 1.
It's Official: Leno Replacing Conan
"We're pleased that Jay is returning to host the franchise that he helmed brilliantly and successfully for many years," Jeff Gaspin, the chairman of NBC Universal Television Entertainment, said in a written statement Thursday. "He is an enormous talent, a consummate professional and one of the hardest-working performers on television."
O'Brien reportedly won't be allowed to take his best-known characters and skits with him when he leaves NBC.
O'Brien is happy with the severance package but is also supplementing staff severance out of his own pocket with a seven-figure sum, manager Polone said Thursday.
Polone said O'Brien wants to return to the air as soon as possible.
"He's doing so well right now it'd be horrible to lose that momentum," he said. "The numbers are so high and its clearly not just about the controversy."
O'Brien, he said, would want to establish a show similar to the "Tonight Show."
Conan Takes on NBC
Leno and O'Brien have both taken shots at NBC and, in some cases, at each other since NBC announced its late-night shuffle. But it has been O'Brien's bitterly comic jabs at the peacock network -- Tuesday night, O'Brien went bilingual in his attacks, saying in Spanish that "NBC is run by brainless sons of goats who eat money and crap trouble" -- that seem to have driven his ratings higher and encouraged passionate fans, including hundreds who held rallies in four U.S. cities earlier this week in support of the host many refer to by the nickname "CoCo."
NBC Universal president and CEO Jeff Zucker defended the network's decision to shift Leno back into the 11:35 p.m. time slot he held for 17 years before O'Brien took over "The Tonight Show."
"From a financial standpoint, this is the right move," Zucker said in an interview with The Wall Street Journal earlier this week. "We didn't want to do it, because we wanted to keep Conan. But we're going to be fine, even paying Conan to go away."
He told the newspaper he was surprised at the "nasty" turn the shake-up had taken.
"We were not surprised that Conan was disappointed in having his show back up a half hour. But we were very surprised and disappointed at how nasty it turned," he said.
Many "Tonight Show" employees lived in New York and worked for O'Brien's former show before moving to California to work on "The Tonight Show."
"The main issue at this stage is how well they're planning on taking care of the people who are out of work, and that's Conan's main concern and that's the focus of all negotiations at this point," Polone told ABCNews.com Tuesday.
But NBC criticized Polone's portrayal of the negotiations, calling it a "PR ploy" and arguing that O'Brien's decisions, not NBC's, will leave his staff jobless.
"It was Conan's decision to leave NBC that resulted in nearly 200 of his staffers being out of work," the network said Tuesday in a written statement. "We have already agreed to pay millions of dollars to compensate every one of them. This latest posturing is nothing more than a PR ploy."
Leno explained his side of the story on his show Monday night.
The former, and now future, "Tonight Show" host said he had tried to avoid doing a show in prime time but was convinced by NBC that it could work and that he would be able to keep his staff of 175.
Four months later, he said, network executives informed him they were canceling his show but told him they wouldn't let him out of his contract because he was still "a valuable asset" to the company.
He said he agreed to host his show at 11:35 p.m. after NBC "almost guaranteed" to him that O'Brien would accept a "Tonight Show" shift to 12:05 a.m.
O'Brien said Tuesday that hosting the "Tonight Show" was a dream of his since he was a child.
"When I was a little boy, I remember watching The Tonight Show With Johnny Carson and thinking, 'Someday, I'm going to host that show for seven months,'" he joked.
With reports from ABC News' Brian Braiker, Ammu Kannampilly, Gregory Croft and Sheila Marikar.