The league said players and staff from last-placed Karpaty Lviv had tested positive ahead of Sunday’s match against Mariupol.
They aren’t showing symptoms and are in self-isolation, Karpaty said, adding that the squad would undergo more testing Monday.
The league is playing its first games since March 15, with Kolos and Desna kicking off the opening match.
There are nine rounds left. The league is due to finish July 19 with a European qualification playoff following 10 days later.
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