The social media company said it is extending restrictions already in place for federal elections in the United States.
Under the new rules, which will also apply in Australia and India, political advertisers will need to be certified.
It's also taking steps to increase transparency. Ads, in the form of "promoted tweets," from the past seven days will be stored in a publicly accessible database showing how much was spent, how many times it was seen and the demographics of the people who saw it.
Facebook and Google have put in similar systems ahead of the EU vote in May, as the U.S. tech companies respond to criticism they didn't do enough to prevent misuse of their platforms by malicious actors trying to sway previous elections around the world.
Hundreds of millions of people are set to vote for more than 700 European Union parliamentary lawmakers.
Twitter defines political ads as those bought by a party or candidate or that advocate for or against a candidate or party.