The Ryman Auditorium responded to Morgan's June appearance -- and comments -- with a statement saying the theater "regrets that people were offended by statements" Morgan made, adding that it "does not control the content presented by people appearing on its stage, nor does it endorse any of the views of, or statements made by, such persons."
Morgan's representative issued a statement to gay rights website Unicorn Booty, saying, "There is no comment. Thank you."
Calls to Morgan's rep by ABC News were not immediately returned.
This is not the first time that Morgan has been accused of homophobic slurs. In a 2009 Carnegie Hall stand-up show, audience members walked out within the first 30 minutes after Morgan again stated that homosexuality was a choice. An audience member told the New York Daily News, "There was a continuous flow of people getting up and leaving throughout the show. Middle-aged women were the most flabbergasted when he started mimicking explicit sex acts on stage."