"Let us be satisfied when our young men are going to college not going to jail," she said to loud cheers and applause. "The movement is not there. Let's continue the movement!"
Sharpton brought the crowd again to its feet with a verbal slap against President Bush.
"The media keeps talking about passing the baton," he said of news coverage about former President Clinton's solid endorsement Wednesday night of Obama. "The only baton that we're going to pass is from George Bush to Barack Obama."
Sharpton said civil rights leaders will go in November to "states that seem to have difficulty counting votes."
"We're going to make sure that the names of African-American voters don't mysteriously disappear," he said. "Not this time! We will not be divided. Not this time!"
Others expressed pride in Obama for being the first black to be nominated for president on a major party ticket.
"I just wish my dad was here to see this," said actress Holly Robinson Peete, whose father, television producer Matt Robinson, marched on Washington.
"He would be so proud of Obama," she said.
"To have it occur on the 45th anniversary of the march on Washington that it was destined to be. It could have never been anyone else," Tyson said.
"My old friend Jane Pitman used to say that when a child is born folks look into its face and ask, 'Are you the one? Are you the one? Well, he is definitely the one," she said.
Other speakers echoed that Obama's candidacy alone isn't enough.
Charles Steele of Georgia's Southern Christian Leadership Conference said Obama is about to make history but the fight isn't over.
"We might quit at this point but we can't quit. We can't let Martin Luther King and other civil rights leaders in the movement down," Steele said. "I challenge you to think about this moment not as a fulfillment of the dream but an important step along the way."
Steele said blacks still have to fight on a daily basis against higher rates of poverty and racism.
Georgia delegate Aaron Johnson told ABCNews.com, "Just because one out of a hundred makes it doesn't make it easy for the other 99."
"While there is a chance and there is more hope for people, we still have a long way to go as a people as a nation," Johnson said, "As long as there is poverty, and there are people who don't have and people who can't have, there's always going to be room for improvement."
ABC News' Jake Tapper, Kate Snow, Karen Travers, John Berman, Tahman Bradley, and Nitya Venkatarman contributed to this report.