"Sen. Clinton misled the voters of Ohio," he said, arguing that Clinton had been duplicitous and double-dealing when it came to claims that she had helped pass the Family and Medical Leave Act, in her push for revotes in Michigan and Florida, and in her claims that her campaign is not promoting the story about Obama's relationship with the controversial Rev. Jeremiah Wright.
The campaign manager also mentioned a recent "60 Minutes" interview in which Clinton said Obama wasn't a Muslim "as far as I know."
"She has shared prayer breakfasts with Sen. Obama and knows without a shadow of a doubt that he is a Christian," Plouffe said. "These kinds of evasions and misleading of voters causes a real 'character gap.'"
For its part, the Clinton campaign released a campaign memo calling Obama "desperate to change the subject," and accusing his campaign of reckless tactics.
"It's not a pretty sight -- it's all part of a pattern of just words," said the Clinton memo, saying Obama "calls for high-minded debates while practicing lowdown politics."
"It's no surprise that Americans are expressing serious doubts about his ability to answer the 3 a.m. call," concluded the Clinton campaign memo.
In light of accusations from the Clinton campaign that there is still much unknown about Obama, Plouffe said, "If Sen. Clinton wants to have a conversation about electability and vetting, we're happy to have it. … The central assertions in her candidacy have proven to be false."
Assailing the Clintons' refusal so far to release any recent tax returns or the names of donors to the William J. Clinton Presidential Library, Plouffe asked, "What are the Clintons scared of? Why won't they release this information? … What's lurking there?"
Michael Elmore contributed to this report.