"Our caucus understands our concern for the rights of the individual member," Pelosi said after a meeting Tuesday with House Democrats, "but also our higher responsibility to our country to uphold a high ethical standard in the Congress of the United States."
Weiner, who has not been charged with or convicted of violating any laws or House ethics rules, had insisted he would remain in office despite the pressure from his colleagues. On Monday, he received a two-week temporary leave of absence from the House to receive "treatment" for an undisclosed condition at an unknown location.
Meanwhile, Weiner's wife returned from an overseas trip with Secretary of State Hillary Clinton early Wednesday morning and met with her husband in person for the first time since the sexting scandal broke. Weiner had told friends he was waiting for her return before making any decision about his political future.
ABC News' Amy Walter, Chris Vlasto, John Parkinson and Marisa Bramwell contributed to this report.