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Stroker was the first actress who uses a wheelchair for mobility to be nominated for and win a Tony.
She told the audience the win was for "every kid who is watching tonight, who has a disability, who has a limitation or a challenge" and every kid "who has been waiting to see themselves" up on that stage.
The New Jersey native, 31, was paralyzed at the age of 2 in a car accident. After her run on "Glee" a few years back, she became the first actress who uses a wheelchair for mobility to appear on Broadway, starring in "Spring Awakening" in 2015.
Then came her nomination and win for "Oklahoma!"
On Sunday night, she also thanked her friends -- and especially her parents -- for teaching her to "use my gifts" to help and inspire others.
"We did it!" she exclaimed to close her speech.
Stroker spoke to ABC News a couple years back about her rise to stardom on Broadway and "turning this limitation that a lot of the world sees and making it into an opportunity."
"The Broadway opportunity, there were a lot of young people who would come and say they didn't think it was possible [performing on that grand stage with a disability], and now I know it is," she said in 2017.
Those words were never truer than Sunday night.