Rowe, 50, married Jackson in 1996 after meeting him while working as a nurse in the office of Jackson's dermatologist, Dr. Arnie Klein. She conceived son Prince Michael I, 12, and daughter Paris Michael Katherine, 11, through in vitro fertilization.
In February 2008, Rowe told British tabloid The Daily Mail that she had been artificially inseminated but would not give details as to whether the sperm belonged to Jackson or another man.
In photographs, the children appear to have distinctly white skin.
The couple were briefly married following Prince's conception but divorced six months after Paris' birth. Rowe gave Jackson custody of the children but sued him in 2006 for breach of contract in an attempt to regain custody. The pair settled out of court for undisclosed terms and Jackson retained custody.
In recent days, some in the media have suggested that while Rowe carried the children she might not be their biological mother.
Rowe told KNBC that she would submit to DNA testing to prove the kids were really her offspring.
Rowe gave Jackson sole custody of the children in 1999. Their divorce was finalized in April 2000, and she agreed to a multimillion-dollar settlement, according to court records. She stopped visiting the children six months later, because it "was not working out for various reasons," according to an appeals court ruling.
Though Rowe does not have custody of the children, in 2006 an appeals court reaffirmed her parental rights.
Those rights, lawyers said, gave her a case to pursue custody of the children, despite Jackson's stated wishes in his will that Katherine get custody.
The surviving parent, even if divorced and with limited contact with the children, usually has the strongest case for custody, lawyers said.
Though Rowe has reportedly not seen much of the children for more than a decade, she would still have "an advantage" should she seek custody, attorney Gloria Allred said.
Jackson's third child, a son named Prince Michael II but better known as Blanket, was conceived and carried by a surrogate mother whose name has never been released.
Rowe would not have a claim to the custody of that child.
Another source close to the Jackson family, told ABCNews.com that a pending custody battle between the family and Rowe "could get ugly."
ABC News' Lauren Pearle contributed to this report.