Megan McAllister's lawyer Bob Honecker told "Good Morning America" today that the young woman last saw Markoff on June 11 when she traveled to Boston to meet with the Suffolk County District Attorney's Office.
"She told him that she planned to attend medical school," Honecker said, "and also she let him know that she did not expect to return to Boston and it would be quite a long period of time, if ever, before she saw him again."
Markoff, himself a medical student at the time of his April arrest, also pleaded not guilty Monday to the armed robbery of another woman as well as kidnapping and weapons charges.
He is accused of the April 14 shooting of Julissa Brisman, 25, at the Boston Marriott Copley Place Hotel where the two allegedly met after Markoff responded to Brisman's advertisement in Craigslist's "erotic services" section.
Markoff is also charged with the April 10 armed robbery of 29-year-old Las Vegas woman named Trisha Leffler in another Boston hotel. He's also been charged with a third attack on a woman in a Rhode Island hotel days after Brisman's murder.
He is being held without bail in the general population at Boston's Nashua Street Jail, where he was at one time in a medical unit under suicide watch.
He spends most of his time staring out the window and watching TV, a Suffolk County Sheriff's Department source told ABC News.
"He is always picking at himself and drawing blood,'' said the source.
Markoff was also "visably upset" and "started to pick at his ear" the day his fiance visited him in jail.
McAllister has testified before the grand jury, and Honecker said she'll continue to fully cooperate with the district attorney's office.
Honecker said McAllister was in New Jersey at the time of the attacks and wasn't aware that her fiance was keeping any of the evidence police say they've found, including underwear from his victims, plastic zip ties and a gun allegedly purchased using another man's identification.
"I think that Megan will always love and support the man she knew," Honecker said, adding that some of the details released in the last several days, particularly at the arraignment, were very disturbing.
"She will await the legal process and she will hopefully abide by what is the determination of time and the court of law as to what is the real Philip Markoff," he said.
At Monday's court appearance, Markoff never made eye contact with his parents, Richard Markoff and Susan Haynes, who were sitting in the packed courtroom Monday just a few rows behind him.
As the charges against his son were read, Richard Markoff rocked back and forth in his seat. Haynes twitched her hands nervously in her lap.
Markoff's brother Jonathan Markoff was also in court alongside his wife, Deanna, whom he held hands with throughout the arraignment.
Asked by ABC News whether he still believed in his son's innocence, Richard Markoff nodded yes.
Brisman's mother Carmen Guzman cried as Markoff made his pleas, and Brisman's sister bowed her head and began to cry audibly when prosectors read the details of the attack during which they said Brisman was "hit on the head before shot at point blank range three times."