A grand jury is already hearing evidence in the assault cases that have been filed, and Lien is expected to make a video statement for prosecutors for the grand jury, authorities said.
Earlier today, Craig Wright, 29, was charged with assault for allegedly hitting and kicking Lien, authorities said.
According to court documents, Wright was part of a group of bikers who hit Lien through the window of his Range Rover, dragged him out of the SUV, and then beat Lien with their helmets and kicked and stomped on him while Lien was on the ground.
Wright pleaded not guilty to the charges. He is currently in jail and not expected to make bail, his attorney told ABC News.
Six people, including Wright, Caldwell and Braszczok, have now been charged for involvement in the Sept. 29 melee.
Wright was with a group of 20 to 30 motorcyclists who were riding on Manhattan's West Side Highway that day, police said. The bikers tried to block off the road to do tricks, cops said, and they surrounded the Range Rover and then slowed down.
Three other bikers have been charged in connection with the case: Christopher Cruz, who allegedly cut Lien off in traffic, sparking the entire incident; Reginald Chance; and Robert Sims.
Wright was identified in video and photos taken at the scene, cops said. He was seen wearing a leather jacket and a black helmet with spikes on it, police said, noting Wright confirmed to authorities that the photos depicted him.
ABC News' Josh Margolin and Michael S. James contributed to this report.