The money will be split equally between two international non-governmental organizations: Save the Children and the International Rescue Committee.
Half the money will fund a program to vaccinate more than 250,000 children against a potential measles outbreak in northern Syria, according to Save the Children. The other half will go toward education, health care, shelter and sanitation to support families -- particularly women and children -- both inside Syria and in neighboring countries who are suffering as a result of the ongoing conflict.
"By allowing us to make their generous donation to Syrian children public, Sacha and Isla are helping highlight the tragedy of the issue today," said Justin Forsyth, CEO of Save the Children. "These are desperate times for Syrian families facing bombs, bullets and torture in Syria. The couple’s donation will save many thousands of lives and protect some of the most vulnerable children."
David Miliband, president and CEO of the International Rescue Committee added, "Sacha and Isla's donation is a great expression of humanity, and a challenge to do more for the most vulnerable. I hope it is an example to many others seeking practical ways of making a difference during the Christmas and New Year season."
Reps for the two actors did not immediately respond to ABC News' requests for comment.