"There is no alliance. Kasich and I made a determination where to focus our energies, where to focus our assets, where to focus our resources," Cruz told reporters while campaigning in Indiana.
Cruz went on to say that Kasich is a "good" and "honorable" man.
Cruz's comments are an apparent attempt to walk back an agreement between the two campaigns to halt Trump in three upcoming primary contests.
The Kasich campaign did not immediately return ABC News' request for comment.
On Sunday night, the Cruz campaign released a statement saying it would "focus its time and resources in Indiana and in turn clear the path for Gov. Kasich to compete in Oregon and New Mexico."
The Kasich campaign said it would "shift our campaign's resources West and give the Cruz campaign a clear path in Indiana" that same evening.