Trump took aim at James in a tweet on Friday night, days after an interview with CNN's Don Lemon aired in which the Los Angeles Lakers star said he felt that Trump was "using sports to kinda divide us."
"Sports has never been something that divides people," James told Lemon. "It's always been something that brings someone together."
Trump also appeared to take sides in the LeBron vs. Jordan debate, saying, "I like Mike!"
Jordan, who owns the Charlotte Hornets, issued a statement Saturday backing James.
"I support LJ," Jordan said in the statement. "He's doing an amazing job for his community."
NBA commissioner Adam Silver, who was at the league's NBA Africa Game on Saturday in Johannesburg, South Africa, commended James for his work on and off the court.
"LeBron James is one of the all-time greatest NBA players and one of the most accomplished athletes," Silver told USA Today. "He runs a very successful media company. He's sent hundreds of students to college and just opened a school in Akron where at-risk students will receive free tuition, meals and transportation.
"I greatly admire his intelligence and business acumen and have enormous respect and appreciation for what he does in his community."
Other players from around the league, including Golden State's Stephen Curry, Minnesota's Karl-Anthony Towns and Utah's Donovan Mitchell, came to James' defense on social media after Trump's tweet.
Lakers owner Jeanie Buss said the franchise "could not be more proud to have LeBron James as part of our Lakers family."
"He is an incredibly thoughtful and intelligent leader and clearly appreciates the power that sports has to unite communities and inspire the world to be a better place," Buss said in a separate statement Saturday. "Those efforts should be celebrated by all."
Lemon, who conducted the interview with James, also weighed in.
The LeBron James Family Foundation and the Akron (Ohio) Public Schools on Monday opened a new elementary school for at-risk children in James' hometown.
The I Promise School is the culmination of nearly a decade of work by James' foundation, with its focus on educating children from challenging situations and backgrounds. The school will begin with two grades, third and fourth, with plans to expand in the coming years.
For those who complete the program, which has been operating for years, James has arranged for free tuition to the University of Akron starting in 2021. He also created a program for parents of the at-risk children to return to complete their high school educations, and he has planned an institute to help prepare high-school-age students for college.
In all, James and his foundation leaders hope that more than 1,200 children will pass through the program and into college by 2029.
"It looks like LeBron James is working to do good things on behalf of our next generation," the statement issued by Stephanie Grisham said, "and just as she always has, the First Lady encourages everyone to have an open dialogue about issues facing children today."
President Trump traveled to Ohio on Saturday. He did not mention James during a campaign rally.
ESPN's Brian Windhorst and The Associated Press contributed to this report.