In an announcement on the airline's blog, JetBlue said it would provide "free seats on its available flights to/from Orlando for immediate family and domestic partners of victims who were killed or injured."
It will also waive fees for customers traveling to or from Orlando who need to make last-minute changes to their travel plans.
"This weekend’s events are felt by all of our 19,000 crewmembers, many of whom live in, work from and travel through Orlando – one of our focus cities – at Orlando International Airport, our Orlando Support Center and JetBlue University," the post said.
"We want to do our part to help the victims of this tragedy, as well as support the Orlando community through this difficult time."
The act was met with thanks on social media. "Heard about your CARE TEAM program flying immediate family members & domestic partners of the victims to Orlando for free. You are amazing. You will absolutely have my business from now on. As an LGBT woman from New York, I say with my deepest gratitude: Thank you," wrote Liz Light o Facebook.
The airline will also make a charitable donation to support victims and their families.
Family members should call 1-800-JETBLUE.