Posing as a potential client, police said they met up with Tichelman to a hotel on July 4 and arrested her.
She appeared in court today on manslaughter and drugs charges but did not enter a plea. Tichelman, who was appointed a public defender, is being held on $1.5 million bail.
The newly uncovered surveillance video shows a woman, who Santa Cruz police say is Tichelman, administering the drugs and never trying to help Hayes or to call authorities when it was clear something had gone terribly wrong, Deputy Chief Steve Clark said.
"She was so callous that in gathering her things, she was literally stepping over the body and at one point stepped over the body to grab a glass of wine and finish the glass of wine," Clark told ABC's San Francisco owned station KGO-TV.
Police said the woman believed to be Tichelman even drew the blinds to the bedroom on the 50-foot yacht before leaving.
Investigators took Tichelman's fingerprints from the wine glass, according to KGO-TV. An obituary posted in the Santa Cruz Sentinel describes Hayes as a husband and father to five children.
"More than anything else he enjoyed spending time with his family at home and on his boat," the obituary said. "His brilliant mind, contagious smile, and warm embrace will be missed and cherished in memories by his friends and family."
The Associated Press contributed to this report.