Sonia Manzano is leaving the show after 44 years, "Sesame Street" confirmed to ABC News.
On the show, Manzano ran a Fix-It Shop with her on-screen husband, Emilio Delgado.
The two-time Emmy nominee and award-winning writer, 65, retweeted her fans' well wishes as she enters the next stage in her career.
She also wrote back thanks to her supporters, but "don't be bummed!"
Manzano has a biography, "Becoming Maria," out later this summer.
Editor’s Note: A previous version of this story incorrectly stated that Sonia Manzano was on “Sesame Street” for 45 years. It was 44.