Israeli forces "temporarily" closed the humanitarian route in the area of Rafah, in southern Gaza, on Tuesday, saying Hamas operatives "opened fire" toward the route.

This is the second time the IDF has closed this route in the past week.

The route is an 8-mile road going from the Kerem Shalom crossing, near Rafah, north near Khan Younis and the humanitarian zone.

Hamas said it will not be attending cease-fire negotiations in Doha, Qatar, on Thursday.

"The movement demands a clear commitment from the occupation to what was agreed upon on July 2, according to the clarifications conveyed by the mediators, and if that happens, the movement is ready to enter into the mechanisms for implementing the agreement," Hamas Political Bureau Member Dr. Suhail al-Hindi told Al-Araby Al-Jadeed TV.

Israeli Prime Minister Benjamin Netanyahu approved the sending of an Israeli delegation to Doha, Qatar, on Thursday to continue negotiations for a cease-fire agreement.

This comes as Netanyahu is receiving pushback internally and externally amid reports he changed the parameters of what he would agree to.

President Joe Biden addressed the rising tensions in the Middle East Tuesday after U.S. officials warned Iran could launch a retaliatory attack on Israel as early as "this week."

Biden told reporters he expects Iran to hold off on carrying out a retaliatory attack against Israel if a cease-fire deal with Hamas is reached.

"That’s my expectation, but we’ll see," Biden said after arriving in New Orleans, LA.

Last week, Biden along with the leaders of Egypt and Qatar jointly called for Israel and Hamas to return to the negotiating table and reach an agreement that would free hostages and end the war in Gaza.

Hamas leaders declined the new set of cease-fire conditions on Sunday, asking for negotiations to resume around what was presented in July.

-ABC News' Justin Gomez