"I always bring up human rights," Biden said, when asked by a reporter in Jerusalem what he would say about Khashoggi's killing to Saudi Crown Prince Mohammed bin Salman, whom U.S. intelligence determined approved the operation that led to the Washington Post columnist's death.
"But my position on Khashoggi has been so clear," he continued. "If anyone doesn't understand it, in Saudi Arabia or anywhere else, then they haven't been around for a while."
Biden said his "views on Khashoggi" have "been absolutely, positively clear."
The president was speaking during a news conference in Jerusalem alongside Israel's prime minister.
As a presidential candidate, Biden had vowed to make Saudi Arabia a "pariah" state over the 2018 murder of Khashoggi, a Saudi dissident who was based in the United States.
U.S. intelligence agencies later assessed that Prince Mohammed, who is also known as "MBS" and who effectively runs the Gulf nation, had approved the operation that left Khashoggi dead.
But with rising inflation and high gas prices weighing on Americans -- and a slew of geopolitical concerns at play -- the president decided to travel to Saudi Arabia and meet with Saudi leaders, including King Salman and the crown prince.
Biden said Thursday that he was traveling to the kingdom to more broadly promote U.S. interests and "reassert" the U.S. role in the Middle East -- noting he was planning to meet with a number of other heads of state while in the country for a regional summit.
While Biden would not commit to bringing up Khashoggi's case with the crown prince, the late dissident's widow said in an interview she had been assured by the White House Biden would do so.
Hanan Elatr Khashoggi met on Tuesday with a White House official in Washington, and on Wednesday, she said in an interview with Spectrum News that the official had assured her that Biden would mention her late husband's name to the crown prince.
ABC News' Chris Donovan contributed to this report.