"The main reason for the call was to say, 'Why are you breaking protocol and tweeting out these photos?'" White House spokeswoman Sarah Huckabee Sanders told ABC News today.
Sanders declined to say whether Trump requested additional official photos of the crowd from the agency.
The retweet on Inauguration Day was of two photos shown side-by-side, comparing Trump's Jan. 20 inauguration crowd to Obama's 2009 inauguration crowd. As part of its policy, the National Park Service does not release official crowd estimates for permitted events.
The retweet was removed last Friday and the National Park Service issued an apology the following day. Employees were also told last Friday to "stand down Twitter activity across the Department temporarily, except in the case of public safety,” according to a statement from an Interior Department official.
The National Park Service runs and maintains the National Mall where crowds gathered to view Trump's inauguration.
ABC News' Jordyn Phelps contributed to this report.