Prior to the high-stakes debate, Trump's campaign organized a hurried press conference to introduce a surprise guest -- a man who claims he had business dealings with the Biden family.
Tony Bobulinski, a self-described global businessman and retired Naval officer, accused the Bidens of misleading the public about their overseas business dealings -- a move intended to shift the public's focus to unsubstantiated and unproven allegations of corruption against the Democratic nominee and his family.
"I have heard Joe Biden say that he has never discussed business with Hunter. That is false," Bobulinski said. "I have firsthand knowledge of this because I directly dealt with the Biden family, including Joe Biden."
With little warning, Bobulinski appeared in a hotel ballroom to speak with the White House reporters who traveled with the president to Nashville, Tennessee, just hours before the candidates took the stage for the second and final debate before Election Day.
The pre-debate event set the stage for a series of attacks Trump leveled against Biden when the debate got underway.
"Somebody just had a news conference a little while ago who was essentially supposed to work with you and your family, but what he said was damning," Trump said. "And regardless of me, I think you have to clean it up and talk to the American people."
Biden shot back: "I have not taken a penny from any foreign source ever in my life."
Bobulinski's emergence came with fewer than 12 days until Election Day. The man who said he had worked to start a business venture with Hunter Biden accused the former vice president of lying about his knowledge of those foreign business dealings. Bobulinski has been advised by a former counsel from the Trump Administration who is currently on the payroll of the Trump Organization, sources told ABC News.
Without fielding reporters' questions, Bobulinski described discussions he held with Hunter Biden beginning in 2015 to partner in a joint venture with a now-defunct Chinese energy firm. Bobulinski pointed to an unverified email from May 2017 -- after Joe Biden left the White House -- which asked if Hunter should be allocated an equity cut on behalf of "the big guy," which Bobulinski says was a reference to Joe Biden.
Discussions surrounding a venture involving Bobulinski and Hunter Biden never resulted in any completed deals or payments from the Chinese energy firm, according to a Wall Street Journal report. The Journal also reported that corporate records related to the dealings show no evidence of Joe Biden’s involvement.
Bobulinski told the reporters his allegations "are corroborated by emails, WhatsApp chats, agreements, documents, and other evidence," including three cellphones he said he would be handing over to Senate Republicans later this week.
Copies of the documents Bobulinski planned to give Congress were obtained by ABC News. ABC News has not verified their authenticity, but they do not show any direct evidence that Joe Biden was aware of, or benefited from any deal.
The Wall Street Journal reported that another partner in the venture, James Gilliar, told the newspaper he was “unaware of any involvement at anytime of the former Vice President."
The Biden campaign immediately issued a full-stop rebuttal of Bobulinski's claims, which it characterized as a "desperate, pathetic farce executed by a flailing campaign with no rationale for putting our country through another four years of hell."
"Joe Biden has never even considered being involved in business with his family, nor in any overseas business whatsoever," said Biden campaign spokesman Andrew Bates. "He has never held stock in any such business arrangements nor has any family member or any other person ever held stock for him."
Trump and his allies have sought to weaponize Hunter Biden's foreign dealings for more than a year. Those controversial endeavors abroad, including in China and Ukraine, did garner criticism from some ethics experts, who said the dealings created an appearance of a conflict.
But no evidence has emerged to suggest that Hunter Biden's private business deals influenced his father's actions or U.S. foreign policy during his time in office. And Joe Biden has said it did not.
During the debate, Biden said that "the only one who has made money from China is this guy," referring to Trump. The Biden campaign's response also highlighted recent reporting in the New York Times that ties Trump and his family to Chinese business endeavors over the years.
The newspaper reported that Trump "spent a decade unsuccessfully pursuing projects in China" and maintains a bank account there.
Trump defended his business interactions in China as something "millions of other people" do, but said he decided not to move forward when he decided to run for president.
"I was a businessman doing business," Trump said. "The bank account was in 2013 -- that's when it was -- it was closed in 2015, I believe … I had an account open, then I closed it."
Since taking office, the president's adult children, Eric and Donald Jr., have continued to operate The Trump Organization in a host of business deals overseas, including in India, Uruguay, the United Arab Emirates and Turkey during their father's presidency.
This report was updated on Oct. 24 to reflect additional reporting.