"He's a war hero because he was captured," Trump said at the event, hosted by Iowa social conservative activist Bob Vander Plaats. "I like people who weren’t captured."
During Perry’s session, a gay man from Ames asked him how a person can push an agenda on him and deny his rights to pursue happiness.
“My belief is that marriage is between a man and women,” said Perry who went on to discuss how he thinks this issue should be up to states to decide and focused on the importance of picking Supreme Court Justices.
“He didn’t answer my question,” said the man.
But the session continued and at one point, Perry brought up Trump’s remarks without being asked about them.
Rubio didn’t have the chance to respond to Trump on stage because he spoke right before Trump, focusing on the threat of ISIS.
“Right now, it's not an issue because this president isn’t arresting any terrorists any more," Rubio said. "He’s not interrogating any terrorists any more. He wants to close Guantanamo.”
Graham carried that theme by looking directly at the crowd and saying: “We’re in a religious war right now. They would kill everyone in this room if they could.”
Although he’s trailing in the polls, Jindal saw the most standing ovations when he spoke late Saturday. Jindal talked about what he said were attacks on the American family and his Christian faith. He also told the audience to give Bernie Sanders credit for being an honest person and admitting that he’s a socialist.
"It’s not just the Democrats," he said. "I’m going to have problems working with some of these Republicans in Washington too."
Besides condemning Trump’s comments, the other nine candidates didn’t attack each other and spent the majority of their time attacking President Obama and at times, Democratic frontrunner Hillary Clinton.
Graham, whose parents died when he was young, got emotional when asked about what they would say seeing him run for president.
"I've been on my knees, and some people helped me to get up," he said. "I feel like I’m on Oprah,”
Luntz responded: "Who does that make me?"
Religion was the overall theme of the day with a Bible on the table next to Luntz. At one point, Rubio read Luke chapter 12, verse 48 from that Bible -- "From everyone who has been given much, much will be demanded" -- and the crowd loved it.
The final speaker, Wisconsin Gov. Scott Walker also touched on religion saying: “That’s something that was part of the founding of this country. That’s something that we should never lose sight of -- protecting religious liberties.”
But, it was no surprise when Walker decided to close his remarks touching on the story of the day, which came from the very stage and seat he occupied.
"I don’t believe in attacking other candidates, but John McCain is an American hero and I will defend him,” said Walker.
ABC’S Alec Goodwin contributed to this story.