Amendola, who suffered the injury in the third quarter of New England's victory Monday over the Buffalo Bills, did not travel with the Patriots to Denver on Saturday, according to reports.
WBZ-TV in Boston first reported that Amendola would not travel with the team.
Amendola was a limited participant in practices Thursday and Friday. The Patriots officially listed him as questionable on their Friday injury report.
Without Amendola and injured No. 1 receiver Julian Edelman (foot), the Patriots (10-0) are going to Denver without two of their top three players on the receiver depth chart.
Furthermore, No. 4 receiver Aaron Dobson was placed on season-ending injured reserve Thursday, and four-year veteran Keshawn Martin has missed the past five games with a hamstring injury. Martin is listed as questionable for Sunday's game.