The pursuit began in Wisconsin after police received reports that there was a man inside the bus with a gun, threatening to kill other passengers.
The chase crossed the state border into Illinois, and ABC affiliate WISN reported that officials put spike strips on the ground to stop the bus in Wadsworth, Illinois.
Greyhound said in a statement Friday night, "The bus with 50 customers onboard was en route from Milwaukee to Chicago when the incident began around 9:50 pm CST. There have been no injuries reported. We are fully cooperating with authorities as they work to get customers to safety."
All of the passengers were evacuated and there is an ongoing investigation by Illinois State Police.
Interstate 94 was shut down in both directions in the area of where the bus stopped.
The identity of the man was not released, nor was his motive.