Ross was going through security at the Louis Armstrong New Orleans International Airport when she said she felt uncomfortable by the way the female TSA agent put her hands between her legs, front and back, she wrote.
Ross described the agent's actions as "over the top," saying that it's "not what was done but how."
"Makes me want to cry," Ross wrote.
She performed at the New Orleans Jazz and Heritage Festival on Saturday.
The TSA said their initial review of the surveillance footage indicates the officers involved in Ross's screening "correctly followed all protocols," the agency told ABC News.
The TSA will continue to investigate the incident.
"TSA is committed to ensuring all travelers are treated with respect and courtesy," spokesperson Jenny Burke said in an emailed statement to ABC News. "TSA is aware of concerns presented by Diana Ross about her screening experience at Louis Armstrong New Orleans International Airport this morning.
"We encourage Ms. Ross to reach out to TSA so we can further explain our procedures, designed to protect travelers from a persistent threat," the statement continued.
ABC News' Christine Theodorou contributed to this report.