The House on Monday overwhelmingly voted to override Trump's veto of the must-pass sweeping defense policy bill known as the National Defense Authorization Act.

The final vote was 322-87, receiving the two-thirds majority it required. There were 109 Republicans who voted to override the veto and 20 Democrats voted to sustain it.

The Senate is expected to hold its own veto override vote later this week.

If the Senate also overrides the president's veto, it will be the first time Congress has successfully rejected a presidential veto during Trump's presidency.

Shortly before the vote, GOP Rep. Mac Thornberry of Texas, the ranking member of the House Armed Services Committee, urged his colleagues to vote for “the exact same bill” they did before, emphasizing that “not a comma has changed.”

“I would only ask that as members vote, they put the best interests of the country first,” Thornberry said. “There is no other consideration that should matter."

The bill initially cleared both chambers of Congress with veto-proof majorities earlier this month. Trump then officially vetoed the bill last week because it didn't include a repeal of Section 230, a law that shields internet companies from being liable for what is posted on their websites by them or third parties. The bill also included a provision that would rename military bases named after Confederates, which Trump opposed.

The $740 billion bill includes pay raises for America's soldiers, improvements in body armor for women, coronavirus relief, military housing improvements and boosted sexual harassment prevention and response measures, among other items. It has passed both chambers of Congress for 59 years straight with strong bipartisan support.

Some Republicans voted to sustain Trump’s veto despite supporting the bill earlier this month.

The defense bill must become law before noon Jan. 3, when the new session of Congress begins, or it will expire.

-ABC News' Mariam Khan

In a statement, Senate Democratic Leader Chuck Schumer, D-N.Y., said he would try to get the House-approved bill to provide $2,000 emergency checks passed in the Senate on Tuesday.

"Following the strong bipartisan vote in the House, tomorrow I will move to pass the legislation in the Senate to quickly deliver Americans with $2,000 emergency checks. Every Senate Democrat is for this much-needed increase in emergency financial relief, which can be approved tomorrow if no Republican blocks it – there is no good reason for Senate Republicans to stand in the way," he said in the statement. "There's strong support for these $2,000 emergency checks from every corner of the country -- Leader McConnell ought to make sure Senate Republicans do not stand in the way of helping to meet the needs of American workers and families who are crying out for help."

Acting Secretary of Defense Christopher Miller pushed back on Biden's claim earlier Monday about transition interference by the Department of Defense in a statement.

"The Department of Defense has conducted 164 interviews with over 400 officials, and provided over 5,000 pages of documents – far more than initially requested by Biden's transition team. DoD's efforts already surpass those of recent administrations with over three weeks to go and we continue to schedule additional meetings for the remainder of the transition and answer any and all requests for information in our purview. Our DoD political and career officials have been working with the utmost professionalism to support transition activities in a compressed time schedule and they will continue to do so in a transparent and collegial manner that upholds the finest traditions of the Department. The American people expect nothing less and that is what I remain committed to."

The House narrowly passed a bill that would increase the amount of money Americans receive in a second round of pandemic relief stimulus payments to $2,000, up from $600.

The final vote tally was 275-134, receiving the two-thirds majority it required under the expedited vote. There were 44 Republicans who voted with Democrats.

Even though Trump supports the measure, a number of Republicans voted against the bill. Its fate remains uncertain in the Senate.

The House vote comes one day after Trump signed the $2.3 trillion spending and COVID-19 relief bill, which included $600 stimulus checks for Americans who make $75,000 or less. Trump initially threatened to hold up the bill last week and said it was a "disgrace." He spent the next several days calling on Congress to pass a bill that included $2,000 stimulus checks instead.

Democrats took up Trump's offer last week and attempted to pass a bill that would send Americans $2,000 checks via unanimous consent but that attempt failed over Republican objections. House Speaker Nancy Pelosi vowed to bring the bill to the floor for a full recorded vote on Monday.

Even though the bill has now cleared the House, at this point it's unclear if the Republican-led Senate will bring the bill to the floor for a vote. Senate Majority Leader Mitch McConnell made no promises in his statement Sunday when he commended the president for finally signing the COVID-19 relief bill into law.

Pelosi called on Trump to put more pressure on his party to back the payments.

"Every Republican vote against this bill is a vote to deny the financial hardship that families face and to deny the American people the relief they need," she said in a statement released Sunday.

Senate Minority Leader Chuck Schumer said Sunday that he would try to pass the legislation in the Senate.

"No Democrats will object. Will Senate Republicans?" he tweeted.

-ABC News' Mariam Khan

With all new senators having been sworn in, Majority Leader Mitch McConnell welcomed the new members on the Senate floor.

"To say the 117th congress convenes at a challenging time would indeed be an understatement. From political divisions to a deadly pandemic to adversaries around the world the hurdles before us are many and they are serious, but there are also plenty of reasons for hope," McConnell said.

The Senate also passed a series of administrative resolutions and standing orders for the new Congress -- including an agreement to Wednesday as a the date to count electoral votes. It's a date already set, but this formalizes it.

In his welcoming remarks, McConnell urged members to remember their oath to the Constitution.

"We gavel in today like 116 prior Senates have gaveled in before us with plenty of disagreements and policy differences among our ranks but all -- all swearing the same oath to support and defend the same Constitution," McConnell said. "All loving the same country and all of us committed to do what we can to leave behind an even stronger nation than the one we've been blessed to inherit."

The Senate stands adjourned until Wednesday.

-ABC News' Allison Pecorin