For the first time since the first televised debate between John F. Kennedy and Richard Nixon in 1960, there will be no live audience at CNN's studio in Atlanta.

The candidates' microphones will be muted unless it is their turn to speak. They will have two minutes to respond to questions and one minute for rebuttals, and there will be a red flashing light to indicate to them their time is about to expire.

The 90-minute program will have two commercial breaks, but Trump and Biden won’t be able to consult with their teams during them. No props or note cards are allowed on stage.

Here's what experts said about the new rules, and how they may help or hurt each candidate.

Several possible vice presidential picks for Trump are in Atlanta for the debate.

Sens. Marco Rubio, R-Fla., Tim Scott, R-S.C., and J.D. Vance, R-Ohio, Rep. Elise Stefanik, R-N.Y., and former Housing and Urban Development Secretary Ben Carson were seen in a picture posted to X by Stefanik.

Trump has repeatedly teased an announcement of who will join him in the 2024 ticket, but he has yet to make a final decision.

Voters across Wisconsin, Michigan, Georgia and Pennsylvania spoke with ABC News about their unhappiness with both candidates and what they're looking to hear from them during the debate.

"I'd like to see what they're going to do for small businesses in this country," said Scranton resident Jennifer Merceau, whose husband owns a masonry company.

Janee Johnson, who works at Toast 'N Jams in Muskegon, Michigan, said the focus should be on doing the "right things for America."

Michael Kordecki, the owner of that restaurant, wishes he could tell the candidates to "be more positive about America, about our future, and about what we can do with or without new people coming into the country."

Read more about these voters here.

With 131 days until Election Day, this faceoff is occurring months ahead of the usual fall timeline.

"The combination of having so many people with doubts about both candidates, coupled with the first debate occurring before either convention, heightens its potential importance," Republican political strategist Whit Ayres told ABC News.

Veteran Democratic strategist James Carville told ABC News: "People's attitudes are very fixed and voters tend to be pretty entrenched. The person that 'wins' ... in this debate is going to be the one that provides voters with some new information."

-ABC News' Isabella Murray and Tommy Barone

Millions of eyeballs will be on Thursday's debate -- here's what to watch for as Biden and Trump face off.

Will any gaffes or knockout punches break through? Conversations on policy are unlikely to break through, but viewers could remember a serious lapse or a zinger that lands.

Will character or policy rule the day? Both sides have lobbed personal attacks -- Biden has dubbed Trump a "convicted felon" and threat to democracy, and Trump has essentially called the president, without evidence, the leader of a crime syndicate. Will the debate stay focused on issues like abortion, immigration and the economy? Or will the two candidates opt for a more scorched-Earth stance?

Typically, a sitting president is debating a governor or senator, with the incumbent's record the main focus. But Trump also spent four years in the White House. Whose record will get more scrutiny?

The debate is happening uniquely early in an election cycle. It remains unclear if any big moments will be washed away by November, or if it'll help set the tone heading into the summer.

The debate is also featuring a novel format -- microphones will be cut off when candidates aren't recognized to speak, and there will be no audience to feed off of. That could deprive Trump of the energy he seeks, helping Biden. But the microphone cutoffs could help "enforce some discipline" on Trump, said GOP strategist David Kochel.

Read more here.