As the debate turned to bans on gender-affirming medical treatments for minors, Christie offered a different view than the three other candidates on stage.

He said it should be a parent's decision, not the decision of the government.

"We should empower parents to be teaching values that they believe in their homes without government telling them what those values should be," he said. "Yet we want to take other parental rights away. I'm sorry, but as a father of four, I believe there is no one who loves my children more than me. There is no one who loves my children more than my wife. There is no one who cares more about their success in health, in life than we do, not some government bureaucrat."

Christie added: "This is not something I favor. I think it is a very, very dangerous thing to do. But that's my opinion as a parent. I get to make the decisions about my children, not anybody else."

Other candidates dove straight into their opposition to gender-affirming care for minors.

DeSantis said, "You do not have the right to abuse your kids." Ramaswamy said his view is that "transgenderism is a mental health disorder," which fact-checkers have challenged.

-ABC News' Alexandra Hutzler

“Father time is undefeated,” DeSantis told the debate audience twice, raising doubts over Trump’s age, at 77 years old, amid intervening attacks from Christie that he avoided the original question.

“He’s afraid to answer … this is the problem with my three colleagues,” Christie said. “They’re afraid to offend.” DeSantis wouldn’t respond to repeated questions by Christie and the moderators on the former president’s fitness for office.

“I’ll concede you’re fit, Ron,” Christie said. “You’re a new generation.”

- ABC News’ Chris Boccia

Addressing Trump's comments in an interview on Tuesday referring to himself as a "dictator" but only on "Day 1," Christie called the former president an "angry, bitter man who now wants to be back as president because he wants to exact retribution on anyone who has disagreed with him."

"Do I think he was kidding when he said was a dictator? All you have to do is look at the history," he said. "That's why failing to speak out against him, making excuses for him, pretending that somehow he's a victim empowers him."

Christie -- who Trump has dismissed as a failure both as a governor and candidate -- charged that the other hopefuls on stage "make it seem like his conduct is acceptable" because they said they would still support him if he was convicted of federal felonies, which Trump denies.

"Let me make it clear: His conduct is unacceptable," Christie said. "He's unfit."

Christie warned to "be careful of what you'll get."

"He will only be his own retribution. He doesn't care for the American people. It is Donald Trump first," he said, eliciting some boos from the crowd.

-ABC News' Meredith Deliso

Banking experts told PolitiFact that DeSantis’ claim about President Joe Biden pushing a “central bank digital currency” was dubious. Even if the system were technically feasible, current U.S. laws would not permit the kinds of monetary surveillance and control that DeSantis described, they told PolitiFact in April. The Federal Reserve is studying the possibility of creating a digital currency. But DeSantis’ remarks overstate the likelihood that such a system is possible, much less likely, to emerge in the United States -- for a variety of technical, legal and political reasons, experts told us.

-Analysis by Katie Sanders of PolitiFact

Can anyone take down Haley? According to 538’s polling with The Washington Post and Ipsos, Republican primary voters have responded favorably to each of Haley’s first three debate performances, and it’s probably not a coincidence that she’s been rising in the polls at the same time. But that could put a target on her back on Wednesday -- and it’s possible that her momentum will stall if she’s perceived to have a bad performance.

Will anyone go after Trump? So far, the debates have mostly consisted of the candidates on stage attacking each other. But that’s an odd strategy considering that their biggest obstacle to the nomination is Trump. In fact, he’s above 50% in 538’s national polling average -- meaning that even if another candidate consolidates all of the non-Trump vote, Trump would still be ahead. That means that someone is going to have to peel support away from Trump in order to win the nomination -- and one way to do that is to attack him in the debates.

Who will perform best on the economy and immigration? According to the latest 538/Washington Post/Ipsos poll, 52% of likely Republican voters said getting inflation or costs under control was one of the top three issues in determining their primary vote; 41% said controlling immigration was one of their top three issues. These will probably be the most important segments to watch at the debate then: If a candidate really aces a question on inflation or immigration, that could go a long way.

Will anyone watch? The ratings have been falling with every debate: While the August faceoff garnered 12.8 million viewers, the September one got 9.5 million and the November one got only 7.5 million. And only 26% of the respondents to our 538/Washington Post/Ipsos poll said they were very likely or absolutely certain to watch the fourth debate. Obviously, the fewer people who watch the debate, the less anything that happens on stage will matter.

-Analysis by Nathaniel Rakich of 538