Haley indirectly swiped at Trump over growing economic hardships and his foreign policies, arguing that he has waffled on support for Ukraine amid Russia's invasion.

She also targeted the former president over his gripes over the 2020 election, arguing, "We can't live in the past, we can't live in other headlines."

-ABC News' Tal Axelrod

Asked why he should be the GOP nominee instead of Trump, Ramaswamy continued going after Trump, like others on stage, responding: "We've become a party of losers."

"We got trounced last night in 2023," Ramaswamy said, referring to Tuesday's disappointments for conservatives in Kentucky, Ohio and Virginia, adding that there needs to be "accountability in our party."

-ABC News' Meredith Deliso

NBC's Lester Holt posed the first question of the night to DeSantis, asking him why he should be the nominee instead of Trump.

"Now if you look where we are now, it's a lot different than we were in 2016 and Donald Trump's a lot different guy than he was in 2016," the Florida governor said, immediately setting a tone on stage in which the various candidates more bluntly criticized the absent front-runner -- something they had not done throughout much of the early primary campaign.

"He owes it to you to be on this stage and explain why he should get another chance," DeSantis continued. "He should explain why he didn't have Mexico pay for the border wall. He should explain why he racked up so much debt. He should explain why we didn't drain the swamp. He said Republicans were going to get tired of winning. What we saw last night, I'm sick of Republicans losing. In Florida, I showed how it's done."

-ABC News' Alexandra Hutzler

DeSantis just cited his large 2022 victory in Florida as a reason why he’s a stronger candidate than Trump. But I looked into this question a few months ago, and I found that the evidence that DeSantis turned Florida into a red state is pretty thin.

-Analysis by Nathaniel Rakich of 538

Ramaswamy, amid a abortion debate, again brought up his idea about "sexual responsibility for men."

"We talked about access to contraception, adoption. Here's the missing ingredient in this movement: sexual responsibility for men," he said. "We live in an era of reliable genetic paternity tests that are 100% reliable, so we can say men deserve more responsibility, so we can tell women, 'We're all in this together.'"

Ramaswamy has discussed this on the trail before but it's not entirely clear what he means or how it would be carried out. He's said it should be codified into law that men have greater responsibility, financially or otherwise, when they are confirmed via paternity test to be a father.

"It's not men's rights versus women's rights," Ramaswamy said on stage. "It's about human rights."

-ABC News' Alexandra Hutzler