Campus police officers at the University of Arizona in Tucson shot "chemical irritant" munitions at a gathering of protesters early Wednesday, the department said.

The university's president, Robert C. Robbins, had asked the campus police and school officials to "immediately enforce campus use policies and all corresponding laws," according to a statement from his office.

The Tucson Police Department and other law enforcement agencies also responded to campus, the university said.

Campus officers closed several blocks of Park Avenue as they responded to the "unlawful assembly," the department said.

Video appeared to show police handcuffing individuals on the ground and protesters forming a human chain facing officers.

"Police deploying chemical irritant munitions," the department said amid a series of updates on social media. "Follow orders of police and disperse immediately. Avoid the area of University Blvd & Park Ave."

-ABC News' Amanda Morris and Kevin Shalvey

As groups of counter-protesters clashed with Pro-Palestinian demonstrators on the UCLA campus early Wednesday, a university official said staff were “sickened” by the violence.

“Horrific acts of violence occurred at the encampment tonight and we immediately called law enforcement for mutual aid support,” Mary Osaka, a vice chancellor, said in a statement.

Osaka aded that the fire department and medical responders were near the scene.

"We are sickened by this senseless violence and it must end,” she said.

-ABC News’ Bonnie Mclean and Kevin Shalvey

The New York Police Department made at least 100 arrests overnight, with most at Columbia University. Arrests were also made at City College of New York, officials said.

Many of those taken into custody are still being processed at police headquarters.

-ABC News’ Aaron Katersky

Clashes between opposing groups of protesters at the University of California Los Angeles included "multiple acts of violence," prompting university officials to ask police to enter the campus, the Los Angeles Police Department said.

Officers responded to assist campus police "to restore order and maintain public safety," the LAPD said.

State and local politicians said they were monitoring the situation, with Los Angeles Mayor Karen Bass calling the violence "absolutely abhorrent and inexcusable."

Gov. Gavin Newsom said his office had been in contact with law enforcement leadership.

-ABC News' Bonnie Mclean, Amanda Morris and Kevin Shalvey

New York City Mayor Eric Adams urged protesters at Columbia University to "walk away" after dozens of demonstrators stormed the campus' Hamilton Hall in what he called a "violent spectacle that serves no purpose."

Adams said the peaceful demonstrations were being co-opted by "professional outside agitators" seeking to "create discord and divisiveness."

"That is why I'm urging every student, every protester to walk away from this situation now. Continue your advocacy through other means," Adams said at a press briefing Tuesday evening. "We will always protect the right to protest, but we must balance that right with the right to keep students in school and our city safe."

"We cannot wait till this situation becomes even more serious. This must end now," he added.

Chief Edward Caban reiterated that protesters who are not Columbia students are working to escalate the situation and said the New York City Police Department is ready to assist the university as needed.

"We have seen the tactics changing in a way that's endangering public safety," he said.

The NYPD showed footage of what Deputy Commissioner for Intel Rebecca Weiner said showed outside actors making barriers inside Hamilton Hall and "escalating" tactics such as scaling buildings and breaking windows. Cameras in the building have also been destroyed, Weiner said.

Individuals who occupied Hamilton Hall could face charges including burglary in the third degree, trespass and criminal mischief, police said.

A Columbia University spokesperson said Tuesday evening that dozens of protesters were in Hamilton Hall.