Columbia University has threatened student protesters with "clear consequences" after protesters occupied a building in an escalating standoff. Students defied a 2 p.m. deadline to disperse Monday.

Students occupying the building face expulsion, the university warned.

"We made it very clear yesterday that the work of the University cannot be endlessly interrupted by protesters who violate the rules. Continuing to do so will be met with clear consequences. Protesters have chosen to escalate to an untenable situation—vandalizing property, breaking doors and windows, and blockading entrances—and we are following through with the consequences we outlined yesterday," Columbia said in a statement.

Students who did not commit to the terms Columbia's proposed agreement to vacate the encampment by 2 p.m. Monday have been suspended.

"Those students will be restricted from all academic and recreational spaces and may only access their individual residence. Seniors will be ineligible to graduate," Columbia said.

"This is about responding to the actions of the protesters, not their cause," Columbia said.

Columbia University said it has limited access to its Morningside campus except to students residing in seven residential buildings, labs and employees who provide essential services.

The school has also limited access into and out of the building to one gate, closing all other campus entry points.

The restrictions will remain in place "until circumstances allow otherwise," Columbia said.

New York Police Department officers said they would not enter the property unless Columbia requests it, or there is an imminent threat.

Virginia Tech said it has cleared an encampment on campus and arrested 82 protesters for trespassing, after refusing to comply with university policies.

"Virginia Tech strongly supports free speech, even when the content of that speech may be disagreeable to some. However, those rights do not extend beyond the point where they interfere with the rights of others, violate our policies, the Code of Virginia, or federal laws and/or create a threat to safety for others," Virginia Tech President Tim Sands said in a statement.

Meanwhile, Virginia Tech Muslim Student Association said in a letter the 91 students faculty and staff were arrested for peacefully protesting.

"As we reach the end of the semester, 7 months after Oct. 7, we are finding our voices still unheard. We are waiting for your support of us and Palestine," the Virginia Tech MSA said in a statement.

-ABC News' Nadine El-Bawab and Kerem Inal

California State Polytechnic University, Humboldt said it has cleared two campus buildings that had been occupied by protesters for over a week and arrested 25 people.

"Those arrested faced a range of different charges depending on individual circumstances including unlawful assembly, vandalism, conspiracy, assault of police officers, and others. In addition, students could face discipline for conduct violations while any University employees arrested could face disciplinary action," Cal Poly said in a statement.

The university called the occupation of the two buildings "criminal activity" and said there were "serious concerns it would spread even further on campus."

"This morning’s enforcement action was determined to be necessary to restore order and to address the lawlessness and dangerous conditions that had developed. What was occurring was not free expression or a protest," the university said in a statement.

The campus will remain closed until May 10.

Linda G. Mills, the president of NYU, posted a statement Friday evening explaining why the school called in the NYPD to break up an encampment on the Greene St. Walkway earlier in the day.

Mills said 14 people who refused orders to leave the area were arrested and the incident was non-violent.

The president said that the police were called in for numerous reasons, including noise complaints from nearby residents and businesses, safety concerns over the crowds of supporters and counter-protesters and escalating threats.

"The encampment had become increasingly untenable for the NYU community and the neighborhood we inhabit," she said.

Mills said three senior administrators spoke with the protesters over the weekend to come to an agreement over shutting down the encampment.

The president said things escalated on Wednesday when a group of people from a May Day march came to the walkway and got into altercations.

The May Day incident and other issues, including threats leveled at NYU administrators, led the school to call the NYPD, according to Mills.

"The university’s senior leadership and I were compelled to conclude that we could not tolerate the risk of violence any longer and that we could not responsibly or in good conscience wait until something drastically worse were to happen in order to act. We needed to bring this to a close," she said.