The appearance, which featured Harry and Meghan talking about their exclusive podcasts with Spotify, was the couple's first since announcing they are expecting their second child and since confirming they will not be returning as working members of Britain's royal family.
Meghan chose a spring look for the appearance, wearing a citrus primavera peplum hem dress by Oscar de la Renta.
Eagle-eyes royal watchers spotted symbolism in Meghan's dress, which retails for $3,490 at Saks Fifth Avenue.
The dress features lemons and California, where Harry and Meghan now live, is the United States' top grower of lemons, according to the U.S. Department of Agriculture (USDA).
The design of the queen's wedding dress for her 1947 wedding to Prince Philip was inspired by Sandro Botticelli's Renaissance-era painting Primavera, according to Holmes.
Prince Harry and Meghan announced a multiyear partnership last year with Spotify that will see the Sussexes both hosting and producing podcasts with the goal of building "community through shared experience, narratives and values," according to Spotify.
The partnership with Spotify will fall under the Sussexes' new production company, Archewell Audio, described by Spotify as an "audio-first production company."
Archewell is the name of Harry and Meghan's new organization, which features both their production and philanthropic work.