Dr. Sean Conley, the president's personal physician, gave Trump the added measure of confirmation he can head back to public events in a memo addressed to press secretary Kayleigh McEnany and released at Trump's permission.
"This evening I am happy to report that in addition to the President meeting CDC criteria for the safe discontinuation of isolation, this morning's COVID PCR sample demonstrates, by currently recognized standards, he is no longer a transmission risk to others," Conley wrote. "Now at day 10 from symptom onset, fever-free for well over 24 hours and all symptoms improved, the assortment of advanced diagnostic tests obtained reveal there is no longer evidence of actively replicating virus."
"In addition, sequential testing throughout his illness has demonstrated decreasing viral loads that correlate with increasing cycle threshold times, as well as decreasing and now undetectable subgenomic mRNA," he added.
Notably, the memo didn't specifically say whether the president had tested negative for the virus.
White House Chief of Staff Mark Meadows in an interview Friday evening on Fox News reacted to the latest memo on Trump's condition, and the fact he can no longer transmit the virus.
"That means just like you would normally think of somebody if you come into contact with them who could potentially pick up the virus, the good news is that's no longer the case with the president," Meadows said when asked if the memo means the president is COVID-free.
He added, "He's remarkably strong. This is a guy who wants to get out and work on behalf of the American people. It's good news indeed. It's consistent with the CDC guidelines, which says that after 10 days you're going to be able to get out and really live your life as normal again."
Meadows did not say if the president is COVID-free. This comes as the White House has refused to say when Trump last tested negative for COVID-19. The White House declined to comment on Saturday.
Trump is expected to hold his first true campaign rally since his diagnosis with COVID-19 on Monday night in Sanford, Florida.
But even before he hits the campaign trail again, the president spoke briefly to supporters from the White House balcony on Saturday afternoon. He only spoke for about 18 minutes to a group of people gathered by right-wing commentator Candace Owens, and her group, called BLEXIT.
Last Thursday, Conley said the president would be able to begin holding public events on Saturday -- 10 days after his diagnosis. That prompted the announcement on Monday's rally, as well as rallies announced for Johnstown, Pennsylvania, on Tuesday and Des Moines, Iowa, on Wednesday.
He spoke to supporters in Michigan on a telerally Saturday night, telling supporters, "I'm feeling really good. I'm actually a little surprised how good I'm feeling. But I would never cancel this call."
ABC News' Ben Siu and Justin Gomez contributed to this report.