The request was made through a Mutual Legal Assistance treaty to the British Home Office, which is the typical process. The Justice Department, notably, did not make a request through Buckingham Palace, but rather decided to ask for the U.K.'s help in compelling the royal's testimony.
Prince Andrew is only a witness and is not the target of the investigation, ABC News has previously reported.
A spokesman for the Southern District of New York declined to comment. The Justice Department also declined to comment.
The U.K. Home Office typically does not confirm or deny the existence of a Mutual Legal Assistance request, except where disclosure is necessary to obtain the cooperation of the witness, according to the British manual for such matters.
The U.S. Attorney for the Southern District of New York, Geoffrey Berman, has previously sought to encourage Prince Andrew's cooperation.
"The Southern District of New York and the FBI have contacted Prince Andrew's attorneys and requested to interview Prince Andrew, and to date Prince Andrew has provided zero cooperation," Berman told reporters on January 27.
Attorneys for Prince Andrew released a statement on Monday arguing that the Duke of York has, on at least three occasions this year, offered his assistance as a witness to the DOJ. The attorneys also criticized the DOJ for going public about their ongoing pursuit of Prince Andrew's cooperation.
"The Duke of York has on at least three occasions this year offered his assistance as a witness to the DOJ. Unfortunately, the DOJ has reacted to the first two offers by breaching their own confidentiality rules and claiming that the Duke has offered zero cooperation. In doing so, they are perhaps seeking publicity rather than accepting the assistance proffered," Prince Andrew's attorneys said in a statement.
"Any pursuit of an application for mutual legal assistance would be disappointing, since the Duke of York is not a target of the DOJ investigation and has recently repeated his willingness to provide a witness statement. It is hoped that this third offer has not been the cause of the most recent leak about the Duke of York," the statement added.
Prince Andrew has long denied any involvement in the wrongdoings of his late friend Epstein, the famed financier and convicted sex offender who was found dead in an apparent suicide in prison while awaiting trial for sex trafficking.
In a November statement, the Duke of York said, "I am willing to help any appropriate law enforcement agency with their investigations."
Sources have told ABC News the investigation of Epstein's alleged co-conspirators is ongoing and has focused on Ghislaine Maxwell, Epstein's former girlfriend and associate.
Prince Andrew may be able to provide information about his interactions with Epstein and Maxwell in New York, the Virgin Islands and London -- three places where Epstein owned property, and where he allegedly took his victims.