House Majority Leader Steve Scalise also stood up to support McCarthy during the debate on the motion to vacate, saying now isn't the time to stop the progress House Republicans have made.

"When we go back to January, as many people have, we knew that it was going to be a narrow majority," Scalise said. "We also knew it wasn't going to be easy. How many of us came here because we thought this job was going to be easy?"

Scalise continued, "One thing we did know is that if we were going to finally start confronting the problems that had been ignored for years and years and years, we had to change the way this place worked. And one thing Speaker McCarthy embraced from Day 1 is to start making those kind of changes to this institution -- opening up the process, allowing members to be more engaged, having amendments come to the floor, single-subject bills, doing appropriations bills."

"Speaker McCarthy has been leading at the top of the level to make sure we have the tools to do our jobs," he added.

Gaetz said earlier this week he'd support Scalise for speaker and believed other Republicans would, too, telling reporters he thinks "very highly" of the No. 2 House Republican.

During the debate, after initially introducing speeches from a few other Republicans who are backing his motion, including Andy Biggs and Bob Good, Gaetz has since used his time to personally respond to each of the pro-McCarthy speeches being made by other lawmakers.

Those responses have sometimes been as brief as simply disputing the common refrain from McCarthy's backers: that moving to oust him is a short-sighted and punitive act that won't help the conference accomplish its goals.

Not so, Gaetz has continually said as he repeats his argument: McCarthy must go.

-ABC News' Adam Carlson

Right before debate began on the motion to vacate, Gaetz was seen trying to use the microphones on the Republican side of the chamber.

But he was blocked by McCarthy allies and aides, who shooed him to the Democratic side of the chamber.

McCarthy is sitting in the second row on the Republican side of the chamber as debate continues.

With debate continuing between those backing McCarthy and the minority in the GOP who have joined Gaetz, Rep. Jim Jordan, R-Ohio, said McCarthy deserves to keep his job -- calling him "rock solid" on several promises Republicans made when they retook majority control of the chamber.

Jordan pointed to several bills that were passed by the House, including border security legislation and rescinding funding for the IRS. Jordan also touted House oversight activities and defended McCarthy's actions to keep the government open and operational for 45 more days.

"I think the speaker has kept his word," he said. "I know my colleagues and friends are saying different. I think he has kept his word ... I think we should keep him as speaker."

In a 45-minute roll call vote, the House moved to oust McCarthy as speaker.

The final vote was 216 to 210 in favor of Gaetz's motion to vacate, with eight Republicans joining Democrats.

"The office of speaker of the House of the United States House of Representatives is hereby declared vacant," said presiding officer Rep. Steve Womack.

It is the first time in U.S. history that the speaker of the House has been booted from the post, putting the chamber in unprecedented territory.