Vice President Mike Pence tested negative for the coronavirus on Wednesday, according to his office.

Pence’s office said on Tuesday that the vice president had gotten a negative PCR test Tuesday afternoon and negative rapid test Tuesday morning -- Pence had also gotten a negative PCR test Monday. Pence's most recent negative result was from an antigen test.

Pence’s office has not responded to questions about what type of test members of the vice president's group attending the debate were using.

Sen. Harris also tested negative for COVID-19 on Tuesday.

-ABC News' Ben Gittleson and Averi Harper

While President Donald Trump resisted typical debate preparations, Vice President Mike Pence has held lengthy mock debate sessions and enlisted the help of a former prosecutor and state attorney general to play his opponent.

Former Florida Attorney General Pam Bondi -- who was also a member of the president's impeachment defense team -- was tasked to play the role of Sen. Kamala Harris in mock debate sessions with Pence in Washington, D.C. before he left for Utah.

Bondi is a career prosecutor who has a history with Kamala Harris -- both served as state attorney generals.

"Kamala was my colleague when we were attorneys general together and she's very smart, she's a seasoned debated, you know she's career prosecutor so prosecutors can debate and they can debate well," Bondi told Fox News.

While top aides privately call Pence "on-message Mike," referring to his more measured, consistent tone, he will be forced to defend the Trump administration's handling of the coronavirus pandemic as the president suggests his contracting COVID-19 was an act of political courage to help him lead the fight against the virus.

Aides plan to use Pence's experience as the head of the White House coronavirus task force to frame him as being on the "front lines" of fighting for the American people on COVID-19.

Former Wisconsin Gov. Scott Walker has also been advising Pence. Walker helped Pence prepare for the last vice presidential debate against Sen. Tim Kaine in 2016.

Walker told Fox & Friends on Wednesday that there's no doubt Pence will face questions on COVID-19, but will focus on how the administration took quick action -- including shutting down China travel.

Walker said Pence will be "calm" but also "emotional and aggressive" during Wednesday's debate.

-ABC News' Rachel Scott and Katherine Faulders

House Speaker Nancy Pelosi told reporters Wednesday that she's been texting with Sen. Kamala Harris about tonight's vice presidential debate.

Her advice to Harris: be yourself.

She also noted that health care will be a big topic tonight and encouraged Harris to beat that drum loudly.

-ABC News' Mariam Khan

Vice President Mike Pence represents a more traditional style of the Republican Party compared to President Donald Trump but has been a loyal second-in-command throughout their first term in office. Pence has been at the forefront, sometimes leading, major policy efforts of the Trump administration, such as the White House's response to the coronavirus pandemic.

The pair did not personally know one another prior to their 2016 race, but their first term has strengthened their bond. Pence has been steadfast in his support for the president through controversy, whether it was a ban on travel into the U.S. from predominantly Muslim countries, Trump's impeachment by the House of Representatives and trial in the Senate, or the decision to resume large, in-person campaign events amid coronavirus.

Read more on Pence's background here.

-ABC News' Justin Gomez

In response to COVID-19 concerns from Kamala Harris’ team, the Commission on Presidential debates has agreed to add additional safety precautions at the debate.

Vice President Mike Pence and Harris will be seated 12-feet and 3-inches away from each other and from the debate moderator, USA Today Washington Bureau Chief Susan Page. As was the case at the presidential debate, there will be no handshakes between the candidates, and while the Commission said there would also be plexiglass separating Harris, Pence and Page, the Pence campaign has objected to using it.

A senior administration official in Pence’s office told ABC News Tuesday that there had been no formal agreement about the plexiglass and that while Harris and Page could use it, Pence doesn’t want to.

In a statement to ABC News, Pence spokeswoman Katie Miller said, "If Sen. Harris wants to use a fortress around herself, have at it."

Though Harris, Pence and Page will not wear masks on stage, everyone else in the debate hall will be required to wear one. Anyone who takes their mask off will be escorted out, according to the rules.

During the presidential debate in Cleveland, members of the Trump family were seen sitting in the audience without masks, even after being asked to put them on.

Harris and Pence will be tested prior to the debate, according to the Commission, which is a change from the presidential debate when the campaigns were responsible for testing their candidates and traveling parties.

“They’ve got to wear a mask, and if they take their mask off they're gonna be escorted out, and I don't care who they are, they'll be escorted out,” said Frank Fahrenkopf, chairman of the Commission on Presidential Debates, in an interview with ABC News.

The vice president has tested negative each day since Friday, and his White House physician has cleared him from having to quarantine, citing that he is not a “close contact” with anyone who’s tested positive, including Trump.

But Pence was present at the Rose Garden ceremony on Sept. 26 when Trump announced Judge Amy Coney Barrett as his U.S. Supreme Court nominee. Eleven of the attendees have since tested positive for COVID-19. He was also in the Oval Office with Trump the morning of his debate, just days before the president tested positive.

Harris tested negative for COVID-19 Monday after being screened in Salt Lake City, where she has been gearing up for the debate, according to an aide.

-ABC News' Justin Gomez and Averi Harper