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One those persons of interest has previously appeared on "Empire," according to a law enforcement official briefed on the investigation.
Police were tracking the two "persons of interest" and were aware of who they were "for awhile." Investigators learned that these two individuals were returning to Chicago on Wednesday from Nigeria and knew they had to move on them. They are both cooperating and have been questioned throughout the day Thursday.
The two persons of interest are U.S. citizens of Nigerian descent, police said.
Smollett was also questioned by police Thursday, the official said. Police do not plan to bring him in for further questioning Thursday night.
"Today Jussie did answer routine followup questions for Chicago Police Department and continues to cooperate," Smollett's representatives said in a statement to ABC News.
A law enforcement official confirms to ABC News detectives are now actively investigating whether the two people of interest played any role in the attack or if it occurred as reported to police.
Neither of the "persons of interest" has been charged with any crime related to the alleged attack, and there is no evidence that they were involved in any attack on the actor, police said.
But a law enforcement official told ABC News that the homes of the two people being questioned were searched overnight by police for possible evidence.
Authorities retrieved shoes, electronic devices and other items they believe could help them determine whether the two people played any role in the alleged assault, the official said.
Nobody has been charged in connection with the attack.
Attorney Gloria Schmidt, who is representing the two persons of interest, told Chicago CBS station WBBM her clients were detained at O'Hare Airport Wednesday evening and were unaware of the attack on Smollett.
"When they first learned what happened to him, they were horrified," Schmidt said. "This is someone that they know, this is someone that they work with, so they don't want to see somebody go through that.
"They’re really baffled why they’re people of interest," she added. "They really don’t understand how [police] even got information that linked them to this horrific crime, but they’re not guilty of it."
Phone records provided by Smollett do show that he was on the phone with his music manager, Brandon Moore, at the time of the attack.
Chicago police used surveillance technology to track the movements of the two individuals using cameras, public transportation records, and corroborating information provided by Smollett, according to a spokesman.
No cameras captured the attack, police said.
"We know who they are and have brought them in for questioning," a Chicago Police Department spokesman said on Friday.
Late last month, Smollett told police he was brutally attacked in what authorities are calling a suspected hate crime.
He was subsequently hospitalized and released, local authorities said.
Smollett told police that the men also allegedly poured "an unknown chemical substance" on him, possibly bleach, and wrapped a rope around his neck, police said. The rope was still around Smollett's neck when police spoke to him, officials said.
And while Chicago Police have told ABC News that Smollett is only being viewed as the victim in this investigation, the actor is still bothered by some chatter online that his story may have changed in any way.
In his first interview since police say he was attacked last month, the singer and actor said he was heartbroken when he found out that people questioned the details of his story.
"I have to acknowledge the lies, and the hate. And it feels like if I had said it was a Muslim, or a Mexican, or someone black, I feel like the doubters would have supported me much more. A lot more," Smollett, 36, told ABC News' Robin Roberts. "And that says a lot about the place that we are in our country right now.
"It's not necessarily that you don't believe that this is the truth, you don't even want to see the truth," he added.
Smollett, who portrays a gay musician on Fox's "Empire," said the attack happened on Jan. 29, at around 2 a.m. as he was leaving a Subway restaurant in Chicago. He told police that two attackers gained his attention by yelling racial and homophobic slurs at him, according to an initial incident report.
He said the attackers put a noose around his neck, poured an unknown substance, likely bleach, on him and used their hands, feet and teeth as weapons in the assault, according to the report, which ABC News obtained last week via a Freedom of Information Act request. Smollett said the attackers also yelled, "This is MAGA country," during the attack -- a reference to President Donald Trump's "Make American Great Again" slogan.
The singer said he sustained injuries to his clavicle bone and bruised ribs during the attack, but some still questioned if he made the story up.
"For me, the main thing was the idea that I somehow switched up my story, you know? And that somehow maybe I added a little extra trinket, you know, of the MAGA thing," Smollett said. "I didn't need to add anything like that. They called me a f----, they called me a n----. There's no which way you cut it. I don't need some MAGA hat as the cherry on top of some racist sundae."
Smollett said earlier reporting by various media outlets claiming his attackers were wearing MAGA hats was inaccurate.
Others suggested that he may have lied in his explanation for being outside at 2 a.m. in sub-zero Chicago weather, but Smollett said "that's ridiculous."
"I've heard that it was a date gone bad, which I also resent that narrative," he said.
"I'm not gonna go out and get a tuna sandwich and a salad to meet somebody. That's ridiculous. And it's offensive," he added.
Smollett also confirmed reports that he was initially reluctant to contact the police or handover his cellphone to help with the investigation.
"They wanted me to give my phone to the tech for three to four hours. I'm sorry but -- I'm not gonna do that," the singer said. "Because I have private pictures and videos and numbers: my partner's number, my family's number, my castmate's number, my friends' numbers, my private emails, my private songs, my private voice memos."
"I don't know what that's gonna be, to hand over my phone for -- and honestly, by then, inaccurate, false statements had already been put out there," he added.
The musician said he's hopeful that police will track down the suspects. He also believes the two potential persons of interest in a photo released by police in the days after the attack are the perpetrators.
"I don't have any doubt in my mind that that's them," Smollett said. "Never did."
But there's no doubt in his mind that the attack was racially motivated.
"I could only go off of their words. I mean, who says, "f----- 'Empire' n-----," "This is MAGA country, n-----," ties a noose around your neck, and pours bleach on you? And this is just a friendly fight?" he asked. "It's unbelievable to me that anything of this has come to this. That every single thing that I have done, every single thing that I have cooperated with, somehow has gotten twisted into being some bull that it's not."
Smollett decided to speak out so people could hear the "truth."
"I think people need to hear the truth," he said. "'Cause everybody has their own idea. Some are healing and some are hurtful, but I just want young people, young members of the LGBTQ community -- young, black children -- to know how strong that they are."
ABC News' Monica Escobedo contributed to this report.