The late-night ABC talk show host is talking about just how excited he is to have worked with the talk show mogul, who will join him Sunday night after the Academy Awards as a special guest on his seventh annual "Jimmy Kimmel Live: After the Academy Awards."
Winfrey will appear in a sketch routine.
On his show, Kimmel described how he felt upon first meeting Winfrey earlier this week.
"About 20 minutes in, I decided I would give my life for Oprah," Kimmel said, jokingly. "If Oprah needed my lungs, she could have both of them. She's like a magic person, she really is."
Kimmel told ABC News correspondent Chris Connelly that he admired Winfrey for her natural ability.
"Just the ease with which she interviews people, and with which she stands in front of an audience. I don't have that ease - I'm almost always uncomfortable," Kimmel revealed.
Being able to work with Winfrey on the special was like "a validation" of his work, Kimmel said, adding that he never dreamed he'd ever have the opportunity to read a script alongside her.
The special show will also include appearances from George Clooney, Martin Scorsese, Helen Mirren, Charlize Theron, Don Cheadle, Edward Norton, Antonio Banderas, Samuel L. Jackson and several other luminaries from Hollywood.
Kimmel's previous post-Oscar special shows have featured participation by Tom Cruise, Ben Affleck, Robert Downey Jr. and Matt Damon, Jon Stewart and Ellen DeGeneres.
Academy Awards host Billy Crystal will join Kimmel to kick off the post-Oscar show, and the band Coldplay will end the night with a Hollywood block party performance on Kimmel's outdoor stage.