They took home the Grammy for Best New Artist, but Macklemore & Ryan Lewis' biggest moment tonight may have been bringing dozens of gay, straight and multiracial couples together to get married live during the 56th Grammy Awards.

During the duo's song "Same Love," which addresses the fight for same sex marriage, Queen Latifah officiated and Madonna also performed during the nuptials.

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"Music has the power to bring people together at the Grammys," Queen Latifah said, introducing Macklemore. "This song is a love song not for some of us but all of us!"

Macklemore came out rapping in front of an altar, with lyrics like "No freedom till we're equal."

Read: Macklemore & Ryan Lewis Win 4 Grammy Awards

Then Latifah walked out, introducing the couples.

"It is my distinct honor to ask the participants to exchange rings," she said.

Madonna came out singing "Open your heart to me" as new husbands hugged husbands, brides hugged brides or husbands hugged brides, including couples of all sexes and colors. The couples walked the aisles to a standing ovation of everyone in the room. Many in attendance had tears in their eyes.

Macklemore & Ryan Lewis also took home Grammy wins for Best Rap Album for "The Heist" and Best Rap Song for for "Thrift Shop."

Watch: Macklemore & Ryan Lewis Talk Addiction

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